Rapid communication: dinucleotide repeat polymorphism of rainbow trout, FGT3(1).

Rapid communication: dinucleotide repeat polymorphism of rainbow trout, FGT5(1).

PoZymorphism. A multiallelic dinucleotide repeat (CA-microsatellite) polymorphism in genomic DNA of rainbow trout ( Oncorhynchus mykiss Walbaum) was detectable by polymerase chain reaction (PCR) and designated FGTS. Source and Description. Sequence was obtained by conventional dideoxy methods using T7 DNA polymerase. The template was isolated from a library of sizeselected (150 to 400 bp) Suu3A...

Rapid communication: variable number of tandem repeat marker, RVF9401, in rainbow trout.

Polymorphism. A variable number of tandem repeat (VNTR; Nakamura et al., 1987) polymorphism was detected in rainbow trout (Oncorhynchus rnykiss Walbaum) using BgZII. The probe, RW9401, was isolated from a genomic DNA cosmid library developed from the RTG-2 cell line (Wolf and Quimby, 1962). Method of Detection. Nylon filters containing rainbow trout genomic DNA digested with BgZII were prehy...

Rapid communication: variable number of tandem repeat marker, RVF9303, in rainbow trout.

Polymorphism. A variable number of tandem repeat (VNTR; Nakamura et al., 1987) polymorphism was detected in rainbow trout (Oncorhynchus mykiss Walbaum) using MspI and BgZII. The probe, RVF9303, was isolated from a genomic DNA cosmid library developed from the RTG-2 cell line (Wolf and Quimby, 1962). Method of Detection. Nylon filters containing rainbow trout genomic DNA digested with MspI o...

Characterization and comparison of microsatellites derived from repeat-enriched libraries and expressed sequence tags.

The construction of high-density linkage maps for use in identifying loci underlying important traits requires the development of large numbers of polymorphic genetic markers spanning the entire genome at regularly spaced intervals. As part of our efforts to develop markers for rainbow trout (Oncorhynchus mykiss), we performed a comparison of allelic variation between microsatellite markers dev...

Short Communication: Effects of Hypoxic Stress on Electrolyte Levels of Blood in Juvenile Rainbow Trout (Oncorhynchus mykiss)